Sylwia Czarnota

Research Associate at The University of Manchester

Contact Info

Phone: +44 7990448699
Blog: https://thesisandpapers.blogspot.com/
LinkedIn: https://www.linkedin.com/in/sylwia-czarnota/
Email:

Experienced in solution state NMR, protein NMR (backbone assignment, relaxation, high pressure NMR), expression and cloning of recombinant proteins, crystallography, electrochemistry. Looking for opportunities in Industry.

Education

The University of Manchester, UK, Manchester Institute of Biotechnology

Doctor of Philosophy (PhD), Biotechnology

2014 - 2018

University of Warsaw, Poland

Master's degree, Chemistry; Bioanalitics

2010 - 2012

University of Marie Curie, Lublin, Poland

Bachelor's degree, Chemistry; Chemistry of bioactive substances and cosmetics, 

2006 - 2010

Research Experience

The University of Manchester, UK and C4X discovery

Research Associate

June 2018 - Present

BBSRC Innovation Fellowship Fund for research collaboration between Academia and Industry. Project based on NMR, crystallography and molecular biology.

The University of Manchester, UK

Early Stage Researcher

June 2014  -  May 2018

MAGnetic Innovation in Catalysis (MAGIC) - Innovative Doctoral Programme (The University of Manchester). Project based of nuclear magnetic resonance (NMR) experiments to understand enzyme dynamics and structural biology. Involved protein expression and purification, crystallography (two X-ray crystal structures solved to 1.3Å) and NMR (two backbone assignments of ternary complexes completed with 97% coverage, relaxation (with model-free analysis) and high pressure NMR up to 2.5 kbar used for dynamic investigations).

University of Warsaw

Master's degree in Chemistry, Bioanalitics

2010 - 2012

Electrochemical and spectroscopic investigations of interactions of oligopeptide cis-platinum derivatives with dsDNA; experimental and theoretical investigations of qualitative and quantitative interactions of novel, potentially anticancer, compounds (peptide-tethered cisplatinum derivatives) with biopolymer dsDNA. Techniques used: electrochemical (linear, pulse, and square wave techniques) and spectroscopic techniques (CD spectroscopy, UV-Vis spectroscopy).

University of Tartu, Estonia

LC-MS method validation on-line course

2017

LC-MS METHOD VALIDATION (P2AV.TK.829), 52 academic hours (52 hours independent learning), 2 ECTS on-line course
Practice-oriented on-line course on validation of analytical methods, specifically using LC-MS as technique.
• The concept, workflow and scope of validation;
• Selectivity and identity confirmation, both via LC and via MS;
• Linearity of signal, linear range, sensitivity and their relation to calibration;
• Precision, trueness, accuracy, analyte stability and their interrelations;
• Limit of detection and limit of quantitation;
• Ruggedness and robustness.

University of Tartu, Estonia

Estimation of Measurement Uncertainty in Chemical Analysis on-line course

2014

Estimation of Measurement Uncertainty in Chemical Analysis (P2AV.TK.652),
on-line course by University of Tartu, result: A (excellent), 26 academic hours (26 hours independent learning), 1 ECTS
•The concept and origin of measurement uncertainty.
•The basic concepts and tools (distribution functions, standard uncertainty, A and B type uncertainty estimates).
•Principles of measurement uncertainty estimation (random and systematic effects and definitions for precision, trueness, accuracy).
•Overview of the measurement uncertainty estimation approaches.
•The ISO GUM modeling approach.
•The single lab validation approach.
•Comparison of different approaches.

Herbapol Lublin S.A., Poland

Summer Internship in Department of quality control

July 2009  -  July 2009 (1 month)
Professional training in Herbapol-Lublin S.A. (a pharmaceutical company), in the Department of Quality Control. Duties: quantity and quality analysis (chromatography - TLC, HPLC, titration, laboratory work).

Skills and Techniques

NMR - solution state NMR, protein NMR (backbone assignment, dynamics: relaxation, high-pressure NMR)

Protein expression, cloning, purification

Crystallography

Microsoft, Linux, TopSpin, CCPNMR Analysis, Sparky, PyMOL, Coot, Phenix

Honours and Awards

BBSRC Innovation Fellowship Fund (IFF)

University of Manchester & C4X Discovery

June 2018 - November 2018

An academic and industrial partnership to exploit methyl transfer enzymes, involves expression, purification, high-resolution X-ray crystallography and NMR investigation of protein-ligand complexes.


1st Biophysical and Biochemical Symposium

ResoN8 FM group

June 2016

Prize for the best short talk.

Publications

1H, 15N, 13C backbone resonance assignments of human soluble catechol O-methyltransferase in complex with S-adenosyl-l-methionine and 3,5-dinitrocatechol

Biomolecular NMR Assignments

December 2016