BBSRC Innovation Fellowship Fund for research collaboration between Academia and Industry. Project based on NMR, crystallography and molecular biology.
MAGnetic Innovation in Catalysis (MAGIC) - Innovative Doctoral Programme (The University of Manchester). Project based of nuclear magnetic resonance (NMR) experiments to understand enzyme dynamics and structural biology. Involved protein expression and purification, crystallography (two X-ray crystal structures solved to 1.3Å) and NMR (two backbone assignments of ternary complexes completed with 97% coverage, relaxation (with model-free analysis) and high pressure NMR up to 2.5 kbar used for dynamic investigations).
Electrochemical and spectroscopic investigations of interactions of oligopeptide cis-platinum derivatives with dsDNA; experimental and theoretical investigations of qualitative and quantitative interactions of novel, potentially anticancer, compounds (peptide-tethered cisplatinum derivatives) with biopolymer dsDNA. Techniques used: electrochemical (linear, pulse, and square wave techniques) and spectroscopic techniques (CD spectroscopy, UV-Vis spectroscopy).
LC-MS METHOD VALIDATION (P2AV.TK.829), 52 academic hours (52 hours independent learning), 2 ECTS on-line course
Practice-oriented on-line course on validation of analytical methods, specifically using LC-MS as technique.
• The concept, workflow and scope of validation;
• Selectivity and identity confirmation, both via LC and via MS;
• Linearity of signal, linear range, sensitivity and their relation to calibration;
• Precision, trueness, accuracy, analyte stability and their interrelations;
• Limit of detection and limit of quantitation;
• Ruggedness and robustness.
Estimation of Measurement Uncertainty in Chemical Analysis (P2AV.TK.652),
on-line course by University of Tartu, result: A (excellent), 26 academic hours (26 hours independent learning), 1 ECTS
•The concept and origin of measurement uncertainty.
•The basic concepts and tools (distribution functions, standard uncertainty, A and B type uncertainty estimates).
•Principles of measurement uncertainty estimation (random and systematic effects and definitions for precision, trueness, accuracy).
•Overview of the measurement uncertainty estimation approaches.
•The ISO GUM modeling approach.
•The single lab validation approach.
•Comparison of different approaches.
NMR - solution state NMR, protein NMR (backbone assignment, dynamics: relaxation, high-pressure NMR)
Protein expression, cloning, purification
Microsoft, Linux, TopSpin, CCPNMR Analysis, Sparky, PyMOL, Coot, Phenix
An academic and industrial partnership to exploit methyl transfer enzymes, involves expression, purification, high-resolution X-ray crystallography and NMR investigation of protein-ligand complexes.
Prize for the best short talk.